Biden signs bill creating National Pulse Memorial and commemorates Pride Month.

As he honors Pride Month at the White House, President Biden on Friday signed a law to designate the site of Pulse, a gay nightclub in Florida where a gunman in 2016 killed 49 people and wounded dozens, as the National Pulse Memorial.

“May we never have to sign — no president ever have to sign — a monument like this,” Mr. Biden said Friday, surrounded by survivors of the shooting, family members of victims and members of Florida’s congressional delegation, among others.

The legislation, which will transform the former site of the popular club into a permanent memorial with a reflecting pool, was adopted by Congress earlier this month.

In remarks at a ceremony after the signing, Mr. Biden is also expected to denounce state legislatures across the country that are working to limit the rights of lesbian, gay, bisexual, transgender, queer and questioning individuals.

Since taking office, Mr. Biden has sought to restore civil rights protections for L.G.B.T.Q. people that were eliminated by his predecessor, Donald J. Trump. On his first day as president, Mr. Biden signed an executive order that combats discrimination on the basis of gender identity or sexual orientation.

The Biden administration has also noted that 14 percent of the president’s appointees identify as lesbian, gay, bisexual, transgender or queer. Pete Buttigieg, the transportation secretary, was the first openly gay cabinet secretary confirmed by the Senate, and is expected to speak on Friday at the White House. Dr. Rachel Levine, the assistant secretary for health in the Department of Health and Human Services, was the first openly transgender federal official to be confirmed by the Senate.

At a ceremony on Friday, Mr. Biden is also expected to announce the appointment of Jessica Stern as a special envoy at the State Department who will work to advance human rights of “LGBTQI+ Persons,” as part of recognizing the month of Pride. The position was created during the Obama administration, but left vacant for the past four years under Mr. Trump. Ms. Stern currently serves as the director of OutRight Action International, an advocacy group.